A371 „PEth from whole blood“

Quantitative PEth measurement from whole blood –
fast. reliable. reproducible. (RUO)

The “PEth from whole blood” test kit is intended for professional users in clinical and forensic laboratories. It enables the quantitative determination of PEth 16:0/18:1 and PEth 16:0/18:2 using LC-MS/MS.

Advantanges
Specifications
Performance Data
Workflow
Order Information
FAQ

Why “PEth from whole blood”?

Designed for medium to high routine throughput, clear decision-making criteria, and seamless integration into existing LC-MS/MS workflows.

LC‑MS/MS‑Kit for 330 Assays

Scalable for routine laboratories and projects with high sample volumes.

Expanded range of analytes

Quantification of PEth 16:0/18:1 and PEth 16:0/18:2.

Easy sample preparation
(1,5 mL tubes)

Compatible with standard laboratory equipment.

3-minute chromatographic separation

With a 3.2-minute injection interval for efficient sequences.

7-point calibrators & 3-point controls

Reliable quantification and quality control in routine operations.

Extended calibrator range

Additional calibrators and controls are available.

Components available separately

Flexible reordering – including calibrators and control solutions.

More information about PEth

Technical Data & Specifications

Analytes

PEth 16:0/18:1 (main analyt)

PEth 16:0/18:2 (additional)

Matrix: Human whole blood

Measurement principle: LC‑MS/MS

Throughput & Cycle Times

Chromatography: 3:00 min

Injection interval: 3:20 min

Kit size: 330 Assays

Calibration & QC

Calibration: 7-point calibrators

Quality controls: 3‑point‑controls

Extended calibration up to 4000 ng/mL (optional)

All components are also available separately.

Sample preparation

Compatible with standard pipettes and common centrifuge technology

Two-step protocol in 1.5 mL reaction tubes

Simple, rapid sample preparation for routine laboratories

Compatibility & Integration

Detailed IFU/method document available (upon request)

Suitable for use on LC-MS/MS systems with standard ESI sources

Can be integrated into LIMS workflows

Performance data

PEth 16:0/18:1

Linearity4,9 – 6373,8 ng/mL
Measuring range
A371-011
A371-011 with A371-080
A371-011 with A371-082		4,9 – 1000 ng/mL
4,9 – 3000 ng/mL
4,9 – 4000 ng/mL
LOD2,1 ng/mL
LOQ4,9 ng/mL
PrecisionCV [%]; n=160
Level 1
Level 2
Leve 3		8%
5%
5%
Truenessabs BIAS [%]
Arvecon 3/25 A
Arvecon 3/25 B		5,5%
6,9%

PEth 16:0/18:2

Linearity4,1 – 6109,4 ng/mL
Measuring range
A371-011
A371-011 with A371-080
A371-011 with A371-082		4,1 – 1000 ng/mL
4,1 – 3000 ng/mL
4,1 – 4000 ng/mL
LOD1,4 ng/mL
LOQ4,1 ng/mL
PrecisionCV [%]; n=160
Level 1
Level 2
Leve 3		6%
5%
5%
Truenessabs BIAS [%]
ACQ 7245%

Workflow

Ordering information

Ordering numberDescriptionQuantity/Unit
A371-APEth from whole blood – Set for Installation330 analysis
ContentMobile Phase, Precipitation solution , Washing solution, HPLC column , Optimization mix,
alibrator set – Level 0 – 6, Control set – Level I – III
A371-BPEth from whole blood – Routine Set/Refiller330 analysis
ContentMobile Phase, Precipitation solution, Calibrator set – Level 0 – 6
Components available individually:
A371-001Mobile Phase for PEth from whole blood650 mL
A371-006Optimization mix for PEth from whole blood2 mL
A371-010Precipitation solution with internal standard for PEth from whole blood50 mL
W001-01Washing solution1000 mL
A371-011Calibrator set – Level 0 – 6 for PEth from whole blood7 x 1 x 250 μL
A371-012Control set – Level I – III for PEth from whole blood3 x 2 x 250 μL
A371-090HPLC column for PEth from whole blood1 pcs
A371-091HPLC column 2 for PEth from whole blood
(optional)		1 pcs
A371-080Calibrator 7 for PEth from whole blood
(optional)		1 x 1 x 250 μL
A371-082Calibrator 8 for PEth from whole blood
(optional)		1 x 1 x 250 μL
A371-081Control IV for PEth from whole blood
(optional)		1 x 1 x 250 μL

Frequently Asked Questions

What is PEth?

Phosphatidylethanol (PEth) is a direct biomarker of alcohol consumption that forms in red blood cell membranes. Measuring it in whole blood allows for an assessment of long-term drinking patterns, unlike short-lived markers.

Who is this test intended for?

For professional users in clinical and forensic laboratories where the concentration of PEth 16:0/18:1 and PEth 16:0/18:2 in whole blood is relevant.

What results does the kit provide?

Quantitative results (LC-MS/MS) for the PEth species 16:0/18:1 and 16:0/18:2, based on a 7-point calibration and accompanying 3-point controls.

Can sample preparation be automated?

We are currently working on automating the sample preparation process. More information will follow.

Questions?

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