{"id":1525,"date":"2025-10-27T18:28:36","date_gmt":"2025-10-27T18:28:36","guid":{"rendered":"https:\/\/www-staging.archimedline.com\/?page_id=1525"},"modified":"2026-05-06T00:02:19","modified_gmt":"2026-05-06T00:02:19","slug":"a371","status":"publish","type":"page","link":"https:\/\/www.archimedline.com\/en\/a371\/","title":{"rendered":"A371 \u201ePEth from whole blood\u201c"},"content":{"rendered":"\n

Why “PEth from whole blood”?<\/h2>\n\n\n\n

Designed for medium to high routine throughput, clear decision-making criteria, and seamless integration into existing LC-MS\/MS workflows.<\/p>\n\n\n\n

\n
\n

LC\u2011MS\/MS\u2011Kit for 330 Assays<\/strong><\/h3>\n\n\n\n

Scalable for routine laboratories and projects with high sample volumes.<\/p>\n<\/div>\n\n\n\n

\n

Expanded range of analytes<\/strong><\/h3>\n\n\n\n

Quantification of PEth 16:0\/18:1 and PEth 16:0\/18:2.<\/p>\n<\/div>\n\n\n\n

\n

Easy sample preparation <\/strong>
(1,5 mL tubes)<\/strong><\/h3>\n\n\n\n

Compatible with standard laboratory equipment.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

3-minute chromatographic separation<\/strong><\/h3>\n\n\n\n

With a 3.2-minute injection interval for efficient sequences.<\/p>\n<\/div>\n\n\n\n

\n

7-point calibrators & 3-point controls<\/strong><\/h3>\n\n\n\n

Reliable quantification and quality control in routine operations.<\/p>\n<\/div>\n\n\n\n

\n

Extended calibrator range<\/strong><\/h3>\n\n\n\n

Additional calibrators and controls are available.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

Components available separately<\/strong><\/h3>\n\n\n\n

Flexible reordering \u2013 including calibrators and control solutions.<\/p>\n<\/div>\n\n\n\n

\n

More information about PEth<\/strong><\/a><\/h3>\n<\/div>\n\n\n\n
<\/div>\n<\/div>\n\n\n\n
<\/div>\n\n\n\n
\n
<\/div>\n\n\n\n

Technical Data & Specifications<\/h2>\n\n\n\n
\n
\n

Analytes<\/strong><\/h3>\n\n\n\n

PEth 16:0\/18:1 <\/strong>(main analyt)<\/p>\n\n\n\n

PEth<\/strong> 16:0\/18:2 <\/strong>(additional)<\/p>\n\n\n\n

Matrix: Human whole blood<\/strong><\/p>\n\n\n\n

Measurement principle: LC\u2011MS\/MS<\/strong><\/p>\n<\/div>\n\n\n\n

\n

Throughput & Cycle Times<\/strong><\/h3>\n\n\n\n

Chromatography: 3:00 min<\/strong><\/p>\n\n\n\n

Injection interval: 3:20 min<\/strong><\/p>\n\n\n\n

Kit size: 330 Assays<\/strong><\/p>\n<\/div>\n\n\n\n

\n

Calibration & QC<\/strong><\/h3>\n\n\n\n

Calibration: 7-point calibrators<\/strong><\/p>\n\n\n\n

Quality controls: 3\u2011point\u2011controls<\/strong><\/p>\n\n\n\n

Extended calibration up to 4000 ng\/mL<\/strong> (optional)<\/p>\n\n\n\n

All components are also available separately<\/strong>.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

Sample preparation<\/strong><\/h3>\n\n\n\n

Compatible with standard pipettes and common centrifuge technology<\/p>\n\n\n\n

Two-step protocol in 1.5 mL reaction tubes<\/p>\n\n\n\n

Simple, rapid sample preparation for routine laboratories<\/p>\n<\/div>\n\n\n\n

\n

Compatibility & Integration<\/strong><\/h3>\n\n\n\n

Detailed IFU\/method document available (upon request)<\/p>\n\n\n\n

Suitable for use on LC-MS\/MS systems with standard ESI sources<\/p>\n\n\n\n

Can be integrated into LIMS workflows<\/p>\n<\/div>\n<\/div>\n\n\n\n

<\/div>\n<\/div><\/div>\n\n\n\n
<\/div>\n\n\n\n

Performance data<\/h2>\n\n\n\n
\n
\n

PEth 16:0\/18:1<\/strong><\/h3>\n\n\n\n
Linearity<\/strong><\/td>4,9 – 6373,8 ng\/mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
Measuring range<\/strong><\/td><\/td><\/tr>
A371-011
A371-011 with A371-080
A371-011 with A371-082<\/td>		4,9 – 1000 ng\/mL
4,9 – 3000 ng\/mL
4,9 – 4000 ng\/mL<\/td><\/tr>
LOD<\/strong><\/td>2,1 ng\/mL<\/td><\/tr>
LOQ<\/strong><\/td>4,9 ng\/mL<\/td><\/tr>
Precision<\/strong><\/td>CV [%]; n=160<\/strong><\/td><\/tr>
Level 1
Level 2
Leve 3<\/td>		8%
5%
5%<\/td><\/tr>
Trueness<\/strong><\/td>abs BIAS [%]<\/strong><\/td><\/tr>
Arvecon 3\/25 A
Arvecon 3\/25 B<\/td>		5,5%
6,9%<\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n\n\n\n
\n

PEth 16:0\/18:2<\/strong><\/h3>\n\n\n\n
Linearity<\/strong><\/strong><\/td>4,1 – 6109,4 ng\/mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
Measuring range<\/strong><\/strong><\/td><\/td><\/tr>
A371-011
A371-011 with A371-080
A371-011 with A371-082<\/td>		4,1 – 1000 ng\/mL
4,1 – 3000 ng\/mL
4,1 – 4000 ng\/mL<\/td><\/tr>
LOD<\/strong><\/td>1,4 ng\/mL<\/td><\/tr>
LOQ<\/strong><\/td>4,1 ng\/mL<\/td><\/tr>
Precision<\/strong><\/td>CV [%]; n=160<\/strong><\/td><\/tr>
Level 1
Level 2
Leve 3<\/td>		6%
5%
5%<\/td><\/tr>
Trueness<\/strong><\/strong><\/td>abs BIAS [%]<\/strong><\/td><\/tr>
ACQ 724<\/td>5%<\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n<\/div>\n\n\n\n
\"\"<\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
<\/div>\n\n\n\n

Workflow<\/h2>\n\n\n\n
\n
\"\"<\/figure>\n<\/div><\/div>\n\n\n\n
<\/div>\n<\/div><\/div>\n\n\n\n
\n
<\/div>\n\n\n\n

Ordering information<\/h2>\n\n\n\n
\n
Ordering number<\/strong><\/td>Description<\/strong><\/td>Quantity\/Unit<\/strong><\/td><\/tr>
A371-A<\/strong><\/td>PEth from whole blood – Set for Installation<\/strong><\/td>330 analysis<\/strong><\/td><\/tr>
Content<\/td>Mobile Phase, Precipitation solution , Washing solution, HPLC column , Optimization mix,
alibrator set \u2013 Level 0 \u2013 6, Control set \u2013 Level I \u2013 III<\/td>		<\/td><\/tr>
A371-B<\/strong><\/td>PEth from whole blood – Routine Set\/Refiller<\/strong><\/td>330 analysis<\/strong><\/strong><\/td><\/tr>
Content<\/td>Mobile Phase, Precipitation solution, Calibrator set \u2013 Level 0 \u2013 6<\/td><\/td><\/tr>
<\/td>Components available individually:<\/strong><\/td><\/td><\/tr>
A371-001<\/td>Mobile Phase for PEth from whole blood<\/td>650 mL<\/td><\/tr>
A371-006<\/td>Optimization mix for PEth from whole blood<\/td>2 mL<\/td><\/tr>
A371-010<\/td>Precipitation solution with internal standard for PEth from whole blood<\/td>50 mL<\/td><\/tr>
W001-01<\/td>Washing solution<\/td>1000 mL<\/td><\/tr>
A371-011<\/td>Calibrator set \u2013 Level 0 \u2013 6 for PEth from whole blood<\/td>7 x 1 x 250 \u03bcL<\/td><\/tr>
A371-012<\/td>Control set \u2013 Level I \u2013 III for PEth from whole blood<\/td>3 x 2 x 250 \u03bcL<\/td><\/tr>
A371-090<\/td>HPLC column for PEth from whole blood<\/td>1 pcs<\/td><\/tr>
A371-091<\/td>HPLC column 2 for PEth from whole blood
(optional)<\/td>		1 pcs<\/td><\/tr>
A371-080<\/td>Calibrator 7 for PEth from whole blood
(optional)<\/td>		1 x 1 x 250 \u03bcL<\/td><\/tr>
A371-082<\/td>Calibrator 8 for PEth from whole blood
(optional)<\/td>		1 x 1 x 250 \u03bcL<\/td><\/tr>
A371-081<\/td>Control IV for PEth from whole blood
(optional)<\/td>		1 x 1 x 250 \u03bcL<\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div><\/div>\n\n\n\n
<\/div>\n<\/div><\/div>\n\n\n\n
\n
<\/div>\n\n\n\n

Frequently Asked Questions<\/h2>\n\n\n\n
\n
\n

What is PEth?<\/h3>\n\n\n\n

Phosphatidylethanol (PEth) is a direct biomarker of alcohol consumption that forms in red blood cell membranes. Measuring it in whole blood allows for an assessment of long-term drinking patterns, unlike short-lived markers.<\/p>\n<\/div>\n\n\n\n

\n

Who is this test intended for?<\/h3>\n\n\n\n

For professional users in clinical and forensic laboratories where the concentration of PEth 16:0\/18:1 and PEth 16:0\/18:2 in whole blood is relevant.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

What results does the kit provide?<\/h3>\n\n\n\n

Quantitative results (LC-MS\/MS) for the PEth species 16:0\/18:1 and 16:0\/18:2, based on a 7-point calibration and accompanying 3-point controls.<\/p>\n<\/div>\n\n\n\n

\n

Can sample preparation be automated?<\/h3>\n\n\n\n

We are currently working on automating the sample preparation process. More information will follow.<\/p>\n<\/div>\n<\/div>\n\n\n\n

<\/div>\n<\/div><\/div>\n","protected":false},"excerpt":{"rendered":"

Why “PEth from whole blood”? Designed for medium to high routine throughput, clear decision-making criteria, and seamless integration into existing LC-MS\/MS workflows. LC\u2011MS\/MS\u2011Kit for 330 Assays Scalable for routine laboratories and projects with high sample volumes. Expanded range of analytes Quantification of PEth 16:0\/18:1 and PEth 16:0\/18:2. Easy sample preparation (1,5 mL tubes) Compatible with standard […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-product.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1525","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"\nA371 \u201ePEth from whole blood\u201c - ARCHImedline<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.archimedline.com\/en\/a371\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A371 \u201ePEth from whole blood\u201c - ARCHImedline\" \/>\n<meta property=\"og:description\" content=\"Why “PEth from whole blood”? Designed for medium to high routine throughput, clear decision-making criteria, and seamless integration into existing LC-MS\/MS workflows. LC\u2011MS\/MS\u2011Kit for 330 Assays Scalable for routine laboratories and projects with high sample volumes. Expanded range of analytes Quantification of PEth 16:0\/18:1 and PEth 16:0\/18:2. 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